DETAILS – Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. (In-vitro Swedish Study) | DrBeen Medical Lectures | Mon, Feb 28, 2022 (Video with References)

arigelab

3 years ago

Content Source/Owner:

Drbeen Medical Lectures

https://www.youtube.com/c/USMLEOnline/

https://www.drbeen.com/

~ 1 hr

Summary:

An accepted and published Swedish study demonstrates for the first time the presence of Pfizer-BionTech vaccine DNA in the cell.

It is reverse transcribed by LINE-1 proteins of the cells. They are not sure if it gets integrated in our DNA or not. Let’s review the details of their method.

#drbeen #koolbeens #COVID

Chapters

00:00 introduction

00:45 Link to the summary

01:00 Review of the reference material

02:19 Cell preparation for the vaccine

03:35 Vaccine addition to the cells

06:00 RT-PCR for find RNA and then DNA

13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA

18:27 Measuring the volume of proteins in the cytosol and the nucleus

22:35 DNA purification and verification

28:41 Results

28:42 LNP enters the liver cells efficiently

29:33 LINE-1 gene is expressed

30:46 ORF-1 found in cytosol and the nucleus

36:01 DNA from the vaccine found in the cell

43:30 Questions not answered

https://youtu.be/GC9qqpwCb7U

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URL list from Friday, Feb. 25, 2022

CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML

https://www.mdpi.com/1467-3045/44/3/73/htm

Frontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics

https://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes

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URL list from Monday, Feb. 28, 2022

CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML

https://www.mdpi.com/1467-3045/44/3/73/htm

LINE-1 protein localization and functional dynamics during the cell cycle | eLife

https://elifesciences.org/articles/30058

Time evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv

https://www.biorxiv.org/content/10.1101/2021.08.20.457104v1

Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues – PubMed

https://pubmed.ncbi.nlm.nih.gov/33958444/

No evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues – PMC

https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8379926/

Overview of Reverse Transcription – Retroviruses – NCBI Bookshelf

https://www.ncbi.nlm.nih.gov/books/NBK19424/

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology

https://www.nature.com/articles/s41564-020-0735-8

Chimeric RNA – Wikipedia

https://en.wikipedia.org/wiki/Chimeric_RNA

Benchtop Automation for Immunohistochemistry (IHC), ISH & Special Stains (SS) – NanoMtrx 300 – YouTube

https://www.youtube.com/watch?v=3IRl0kbR9-o

Schrödinger’s cat – Wikipedia

https://en.wikipedia.org/wiki/Schr%C3%B6dinger%27s_cat

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Disclaimer:

This video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. The content provided in this video is for informational and educational purposes only.

Please consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. No information in this video should ever be considered as a substitute for advice from a healthcare professional.

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